Method for qualitative determination of 2-deoxystreptamine.
نویسندگان
چکیده
We have developed a qualitative method to assay for the presence of 2-deoxystreptamine in hydrolysates of crude aminoglycoside preparations using a 2-deoxystreptamine-requiring idiotrophic mutant. The assay involves (i) incubation of a 2-deoxystreptamine-requiring mutant with 1 mg of a hydrolyzed preparation from a crude unknown antibiotic mixture per ml, and (ii) examination of the resultant incubation mixture for production of antibiotic(s) by disk assay.
منابع مشابه
Biochemical studies on 2-deoxy-scyllo-inosose, an early intermediate in the biosynthesis of 2-deoxystreptamine. I. Chemical synthesis of 2-deoxy-scyllo-inosose and [2,2-2H2]-2-deoxy-scyllo-inosose.
A practical preparative method for 2-deoxy-scyllo-inosose, the earliest key intermediate leading to 2-deoxystreptamine, was devised as a prerequisite to more detailed biochemical studies on the biosynthesis of 2-deoxystreptamine. [2,2-2H2]-2-Deoxy-scyllo-inosose was also synthesized through a modified Ferrier reaction.
متن کاملIsolation of an intermediate of 2-deoxystreptamine biosynthesis from a mutant of Bacillus circulans.
Eight 2-deoxystreptamine-negative (DOS-) mutants were isolated from various strains of Bacillus circulans, normally producing butirosin, xylostasin and ribostamycin. These mutants were classified into two groups (converter and secretor) by the simple method of cosynthesis using agar plate culture. One of the cosynthetic pairs, strain S-11, an intermediate of DOS biosynthesis, S-11-P, was isolat...
متن کاملGenetic analysis of new 16S rRNA mutations conferring aminoglycoside resistance in Mycobacterium abscessus.
OBJECTIVES We studied the development and fitness cost of 2-deoxystreptamine aminoglycoside resistance of Mycobacterium abscessus. METHODS Spontaneous 2-deoxystreptamine aminoglycoside-resistant mutants were selected and the frequency of their appearance was determined. The 3' part of the rrs gene was sequenced to characterize mutations. Additionally, we determined the MICs of aminoglycoside ...
متن کاملEfficient preparation of a 1,3-diazidocyclitol as a versatile 2-deoxystreptamine precursor.
A synthesis route toward 2-deoxystreptamine, a common structure in many of the clinically important aminoglycosides, is presented. Starting from p-benzoquinone and cyclopentadiene, 2-deoxystreptamine is synthesized with key steps involving Pd(0)-catalyzed rearrangement, a retro-Diels-Alder by flash vacuum thermolysis, and Yb(III)-directed regioselective epoxide opening. The obtained diazidocycl...
متن کاملStructure elucidation of an intermediate of 2-deoxystreptamine biosynthesis.
The structure of a 2-deoxystreptamine (DOS) precursor named S-11-P, which was isolated by using a DOS negative mutant derived from a xylostasin-producing strain of Bacillus circulans B15M, has been elucidated as (1 L)-1,3,5/2,4,-5-aminocyclohexanetetrol by comparison with an authentic specimen, which was synthesized from kanamycin A.
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 14 1 شماره
صفحات -
تاریخ انتشار 1978